《植物生理学报》 2014, 50(9): 1401-1405

高粱浅绿叶突变体sll1的农艺性状和生理生化特性

张灵敏^1,2, 吕文彦^1,*, 张丽霞^2,*

¹沈阳农业大学农学院, 沈阳110866; ²辽宁省农业科学院, 沈阳110161

通信作者：吕文彦；E-mail: lwyzxr@163.com, zhanglx993@sohu.com；Tel: 13909823038, 024-31021081

摘　要：

通过EMS诱变野生型高粱BTx623种子获得浅绿叶突变体sll1 (Sorghum Light-green Leaf 1)。以野生型BTx623为对照, 进行成熟期农艺性状调查和苗期生理生化指标测定。结果表明, 突变体sll1茎粗和穗柄长与BTx623相比没有显著差异, 而株高、穗长、粒数和千粒重均低于BTx623, 存在极显著差异, 且抽穗期延迟15~20 d。苗期叶片叶绿素测定结果显示, 突变体sll1叶绿素a、叶绿素b和总叶绿素含量均极显著低于BTx623, 其中叶绿素b含量只有野生型的1/10, 推测该突变体是叶绿素b减少型突变体。突变体sll1蛋白总量显著低于BTx623, 但脯氨酸含量以及超氧化物歧化酶、过氧化物酶和过氧化氢酶活性均极显著高于BTx623, 说明突变体sll1具有较强的渗透调节能力和清除活性氧的能力以维持自身正常生长。

关键词：高粱; 浅绿叶突变体; 农艺性状; 生理生化特性

收稿：2014-04-25   修定：2014-07-30

资助：国家现代农业产业技术体系任务和高粱重要抗性资源鉴定筛选与种质创新(CARS-06-01-06)。

Agronomic Traits and Physiological-Biochemical Characteristics of Light- Green Leaf Mutant sll1 of Sorghum bicolor

ZHANG Ling-Min^1,2, LÜ Wen-Yan^1,*, ZHANG Li-Xia^2,*

¹College of Agronomy, Shenyang Agricultural University, Shenyang 110866, China; ²Liaoning Academy of Agricultural Sciences, Shenyang 110161, China

Corresponding author: LÜ Wen-Yan; E-mail: lwyzxr@163.com, zhanglx993@sohu.com; Tel: 13909823038, 024-31021081

Abstract:

A sorghum light-green leaf mutant, sll1, was isolated by EMS treating BTx623 seeds. The agronomic traits were surveyed in mature period and the physiological-biochemical characteristics were studied in seedling stage between the wild type BTx623 and sll1. Compared with BTx623, the stem diameter and spike stem length were no significant difference in sll1. However, the plant height, spike length, grain yield and 1 000-grain weight of sll1 were significantly lower than those in BTx623. In addition, the heading date delayed 15–20 days. The contents of chlorophyll a, chlorophyll b and total chlorophyll of mutant sll1 were extremely lower than those in BTx623, especially the content of chlorophyll b (1/10). So, sll1 mutant was presumed the chlorophyll b reduction mutant. Although the protein content of sll1 was obviously lower than that in BTx623, the proline content and the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) of sll1 were significantly higher than those in BTx623, indicating that the mutant sll1 had the strong abilities to adjust osmosis and scavenge active oxygen in order to maintain their normal growth.

Key words: Sorghum bicolor; light-green leaf mutant; agronomic traits; physiological-biochemical characteristics